The DNA fragment above has an overhanging 3-prime end. It : 356029.
The DNA fragment above has an overhanging 3-prime end. It is reacted with T4 DNA polymerase, Mg2 and a mixture of dNTPs. Which of the following statements is correct? (A) It cannot be blunt-ended as DNA polymerases cannot add nucleotides to recessed 5-prime ends. (B) An equal amount of 5-prime end-filled and 3-prime cut back molecules obtained (C) The 3-prime overhang is cut back to the 5-prime end. (D) The 5-prime end is end-filled up to the 3-prime end. (E) t can be blunt-ended but only if the polarity of the strands is reversed. are
The DNA fragment above has an overhanging 3-prime end. It : 356029
