The DNA fragment has an overhanging 3-prime end. It reacted : 356030

The DNA fragment has an overhanging 3-prime end. It reacted : 356030.

The DNA fragment has an overhanging 3-prime end. It is reacted with T4 DNA polymerase, Mg2+ and a mixture of dNTPs. Which of the following statements is correct?

(A) It cannot be blunt-ended as DNA polymerases cannot add nucleotides to recessed 5-prime ends.

(B) An equal amount of 5-prime end-filled and 3-prime cut back molecules are obtained.

(C) The 3-prime overhang is cut back to the 5-prime end.

(D) The 5-prime end is end-filled up to the 3-prime end.

(E) It can be blunt-ended but only if the polarity of the strands is reversed.

 

The DNA fragment has an overhanging 3-prime end. It reacted : 356030

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